Antrodia species explanation - liquid culture (white mycelia)
Liquid culture (white mycelia)
Under liquid fermentation and fermentation, the fermentation conditions were 28 °C, 100 rpm, initial pH 5.0, and foam as the immobilized carrier. The yield of extracellular polysaccharide was up to 0.21 mg/ml on the 14th of fermentation, and the yield of GABA was up to 9.3 mg. /ml. Immobilized cells were cultured in fresh medium for repeated fermentation. At a culture period of 7 days, EPS and GABA concentrations were 0.14 mg/ml and 9.3 mg/ml, respectively, and the immobilized cells were reused 5 times. When alginate was used as an immobilized carrier and liquid fermentation was conducted under the same culture conditions, GABA production was the best on the third day at 8.6 mg/ml, and on the fifth day, the most EPS production was obtained, 0.33 mg/ml. . When the immobilized cells were repeatedly used in fresh medium and cultured for 3 days, the highest concentrations of EPS and GABA were 0.32 mg/ml and 9.6 mg/ml, respectively, and the immobilized cells could be reused. 3 times. Fermentation of immobilized bacterial cells in different fermentation units, with a culture period of 7 days, the concentrations of EPS and GABA obtained were: 2.8 liters of 0.18 mg/ml of Fernbach flask, and 8.3 mg/ml, 7.0 liter of foam. Column fermentor 0.17 mg/ml and 16.0 mg/ml. Compared with non-immobilized bacteria, EPS and GABA production in the bubble column fermenter was 0.14 mg/ml and 14.0 mg/ml. The use of immobilized bacteria in the bubble column fermenter was more beneficial to the fermentation broth. GABA generation.
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